FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18745478
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26250.098.589016
ACGACGC27400.094.979025
CACGACG28500.091.3138964
GACGCTC38400.067.206237
TACACGA44200.059.5840152
CTACACG49600.053.9993061
ACGCTCT48050.053.4073038
ACACGAC50800.052.0854533
CGCTCTT62750.041.5895469
TCTTCCG80900.07.365779410-14
CTTCCGA86300.07.190488310-14
TTCCGAT102650.06.200560610-14
TCCGATC102900.06.101151515-19
TAACCCT761450.05.8930336
CTAACCC813950.05.7266035
CCGATCT114450.05.70080315-19
AACCCTA821500.05.6697591
GTTCCGA30708.5561624E-85.66704565
ACCCTAA836850.05.63522342
CCCTAAC845800.05.5881393
Produced by FastQC (version 0.11.5)