FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18437007
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10100.089.720979
TGCGCGA28550.081.710461
TTTGCGC31500.079.165671
ACGACCG30350.077.587231
CGGTACG30550.076.121791
CGCCGAA28300.075.713671
GTGCGAC31000.074.544991
GCGCTAG34550.069.425481
GTACCGT40500.068.378318
GACGCGT32500.067.9542541
TTACCCG36000.067.644861
CGAACGC34300.067.3730241
CCGCTAT36300.065.67561
GTTACCG29350.065.2087558
ACGCCGA35400.065.072931
CGTTGCG15500.065.0128257
AACGCGT25700.064.599293
GGCGACT36350.064.3781741
CGCGGTA37000.063.6424981
ATAACGC34150.063.3861121
Produced by FastQC (version 0.11.5)