FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15717008
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10700.086.722199
TGCGCGA24950.080.608011
CGCCGAA24350.077.188071
TTTGCGC27950.076.927491
ACGACCG23950.072.064691
GTGCGAC26700.071.216091
CGGTACG25200.070.8117451
GCGCTAG27050.068.94281
GACGCGT21850.068.280191
GTTACGC25000.066.114838
GTACCGT34700.065.8089758
GCGCGTA27350.065.245191
TTACCCG32500.064.582451
GGCGACT30950.063.7997741
CGAACGC27250.063.6059651
ATAACGC29450.063.5727041
TAGGCGC31300.063.5536651
TCACGCG21200.063.2615668
CCGCTAT31050.063.123231
GCCGGAT31700.062.982431
Produced by FastQC (version 0.11.5)