FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19460931
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1860876195.62112418979338No Hit
NAGTAGAG3343771.71819631856256No Hit
AAGGAGAG626230.3217883049891087No Hit
AAGTCGAG604050.3103911112988377No Hit
AAGTAGCG357540.18372194012711932No Hit
AAGCAGAG353770.18178472550979188No Hit
ACGTAGAG346760.1781826367916314RNA PCR Primer, Index 17 (100% over 8bp)
AAGTTGAG340820.1751303676067707No Hit
AAGTGGAG332940.17108122936153466No Hit
AAGAAGAG320910.16489961348714507No Hit
AGGTAGAG227140.11671589606889825No Hit
AAGTAGAA219600.11284146683424344No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)