FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19238828
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1834385695.34809500869802No Hit
NAGTAGAG3501291.8199081565675415No Hit
AAGTCGAG618180.3213189493663543No Hit
AAGGAGAG600080.3119108918692968No Hit
ACGTAGAG395380.20551147918158008RNA PCR Primer, Index 17 (100% over 8bp)
AAGCAGAG373990.19439333830522318No Hit
AAGTAGCG361430.18786487409732028No Hit
AAGTTGAG331430.17227140863258406No Hit
AAGAAGAG318670.16563898798824958No Hit
AAGTGGAG318520.1655610206609259No Hit
AGGTAGAG262800.13659875747108918No Hit
AAGTAGAA215120.11181554302580178No Hit
AACTAGAG208010.10811989171065929No Hit
CAGTAGAG194020.10084813898227066No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)