FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18859435
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12350.085.026339
TTTGCGC31550.078.830191
CGGTACG27400.070.539591
CGCCGAA28450.069.987091
TGCGCGA26800.069.941661
GCGCTAG32050.069.863111
CGAACGC34450.068.8068541
ATAACGC35050.068.669441
TTACCCG35400.068.196541
GTGCGAC32600.068.013251
TCGTACG37200.065.216028
GCGCGTA32000.064.730051
ACGACCG26750.064.6192861
TAGGCGC34600.064.0818561
CCGCTAT36550.063.8557781
CGCGTGA33300.063.7362251
TACGAAC41050.063.4296951
GCCGGAT36050.063.3252141
GTTACGC31250.063.2652178
GGCGACT34950.062.6053851
Produced by FastQC (version 0.11.5)