FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18859435
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC24100.091.125895
CGACGCT24700.087.444886
CACGACG26050.084.58284
GACGCTC36250.059.982547
TACACGA41650.053.4388432
ACGCTCT44300.050.554868
CTACACG45200.049.9659161
ACACGAC51600.043.403873
CGCTCTT58050.038.455759
CGTTGAG26051.5375917E-86.4531821
CGCAAAG30104.7550202E-85.82771831
TAACCCT685900.05.8013176
ACCGGGT23001.9760306E-55.72003171
CCGTAAT26851.1884549E-65.7164811
AACGGGG34654.6966306E-95.695271
CTAACCC730050.05.54977565
CGATGGG38351.546141E-95.52696131
TCTTCCG101100.05.521014710-14
CCTAACC756500.05.43238264
ACCCTAA760650.05.3946752
Produced by FastQC (version 0.11.5)