FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18847507
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12900.091.03329
TTTGCGC30200.080.2857061
TGCGCGA28400.079.924821
CGCCGAA30400.075.151451
TTACCCG34750.074.439181
GTGCGAC30900.073.93541
CGGTACG29000.069.122621
GCGCGTA32850.068.649181
TCGTACG35100.068.152628
CGAACTA38150.067.998191
ACGACCG30400.067.878731
GCGCTAG32400.067.55551
ATAACGC35000.065.906091
GTTACGC29000.064.2407768
TACGAAC36850.063.9973141
GACGCGT29850.062.2164841
AACGCGT23350.061.7778323
TAGGCGC34850.061.7489171
CGACACG38300.061.18961
GCCGGAT37250.061.133811
Produced by FastQC (version 0.11.5)