FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18847507
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22100.079.382476
ACGACGC23000.077.536975
CACGACG25550.069.798444
GACGCTC34550.050.567527
TACACGA36550.048.6615072
ACGCTCT40750.043.0513738
CTACACG41950.042.544621
ACACGAC50400.035.238883
CGCTCTT53050.033.3434529
AACGGGG34556.9849193E-105.9278441
TAACCCT679700.05.7060826
CGCAAAG31159.613905E-85.6355971
CTAACCC721500.05.54631145
GTTCCGA47253.6379788E-125.52334555
CCTAACC746750.05.4461454
CCCTAAC762300.05.3919233
AACCCTA736800.05.36081651
ACCCTAA754700.05.33857632
CGCATAG18000.0057178515.28272061
ATCGGGT18550.0081304965.126091
Produced by FastQC (version 0.11.5)