FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21249866
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13300.087.213579
TGCGCGA33350.078.412141
TTTGCGC33650.074.239851
CGGTACG31350.072.696441
GCGCTAG35850.071.110281
CGCCGAA33450.070.316281
ACGACCG31850.069.032431
TTACCCG42700.066.545371
ATAACGC39350.066.27041
GGCGACT40450.065.3711551
TCGTACG40850.065.308768
GTGCGAC37100.064.382811
CGAACGC40850.063.300521
GTTACGC33200.063.10678
TAGGCGC40400.062.559151
GACGCGT34950.062.491381
CGCGGTA41700.062.0102231
GCGCGTA36300.061.9783741
CCTTACG42100.061.9415741
TCCGCGA31800.061.5534448
Produced by FastQC (version 0.11.5)