FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19498627
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA29450.081.070631
ACGACCG27750.080.51181
CGGTACG29200.076.263731
GTGCGAC34350.076.0952151
TTACGCG11950.075.226559
CGCCGAA30100.074.95371
TTTGCGC29450.074.624651
GCGCTAG36400.069.6031951
GGCGACT40000.066.259441
GACGCGT32900.065.6896441
ATAACGC36550.065.522061
CGAACGC36100.064.923051
TTACCCG38450.064.562991
AACGCGT24750.063.2877273
CCGCTAT35450.063.2300031
GCGCGTA31300.062.2827841
TAGGCGC36050.061.570031
TCGCGAT22450.061.0328878
GTACGCT28050.060.995478
CGCGGTA35800.060.572361
Produced by FastQC (version 0.11.5)