FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18820470
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13650.087.6298149
TTTGCGC31200.081.6961061
ACGACCG26750.078.085921
TGCGCGA29150.074.6634751
GCGCTAG33500.071.2907941
CGGTACG30150.070.733641
ATAACGC33500.069.328671
CGTTGCG15000.069.110717
GTGCGAC33000.069.051191
CGCCGAA29800.068.623411
GTTACGC30800.068.257478
TCGTACG36250.067.994518
TTACCCG36400.066.6142651
GCGCGTA30650.066.0054551
TCGACGG32500.063.5965581
TCACGCG26350.063.552748
GACGCGT28400.063.005471
AACCGCG36450.062.3151131
GGCGACT38650.061.9804731
TAGGCGC37300.061.874081
Produced by FastQC (version 0.11.5)