FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18820470
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24050.094.6505666
ACGACGC26750.085.9091645
CACGACG28600.080.857344
GACGCTC36750.062.3359227
TACACGA39400.060.3392752
CTACACG46800.051.426271
ACGCTCT44900.051.3439648
ACACGAC53850.043.885023
CGCTCTT60000.038.3009649
CGTAGAG29901.8189894E-116.87137751
AACGGGG35351.1295924E-95.8121
TAACCCT696450.05.80835156
GTTCCGA48800.05.79360345
CTAACCC736150.05.5836835
AACCCTA752600.05.55734871
TCTTCCG99900.05.51486710-14
CGTTGAG24053.7871134E-55.49179651
CGATGGT50051.8189894E-125.42443661
ACCCTAA776150.05.40478852
CCCTAAC779450.05.37556363
Produced by FastQC (version 0.11.5)