FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23117074
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2237418896.78641855798878No Hit
NGTCCAGA2224650.9623406491669317No Hit
GGGCCAGA481720.20838277370224279No Hit
GGTCCAGC427900.1851012805513362No Hit
GGTGCAGA330340.14289870768246882No Hit
GGTCCCGA321300.13898817817514447No Hit
GGTCCAGG306770.13270278063737653No Hit
TGTCCAGA298530.12913831568822248No Hit
GGCCCAGA286380.12388246021101113No Hit
GGTCGAGA282310.12212185677131977No Hit
GGTTCAGA281330.12169792768756116Illumina DpnII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)