FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21267605
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG2041496895.99091199972916No Hit
NAGTAGAG1629410.7661464466732385No Hit
AAGGAGAG985260.46326796082586635No Hit
AAGTGGAG615370.2893461675632964No Hit
AGGTAGAG544860.25619245796600043No Hit
AAGTCGAG530760.24956265644392023No Hit
ACGTAGAG360770.1696335812142458RNA PCR Primer, Index 17 (100% over 8bp)
GAGTAGAG335110.1575682828414389No Hit
AAGTTGAG332740.156453911947302No Hit
AAGTAGAA330370.15533954105316514No Hit
AAGAAGAG326570.15355278603303005No Hit
AAGTAGGG322710.15173781909152442No Hit
AAGTAGCG315550.1483711964746383No Hit
CAGTAGAG279800.13156159332468326No Hit
AAGCAGAG267660.12585338123404116No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)