FastQCFastQC Report
Wed 8 May 2024
EGAF00005486514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20794248
Total Bases166.3 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGCGA2009019996.61421273806103No Hit
NATAGCGA2351251.1307213417864401No Hit
CATAGCGC412390.19831926598163108No Hit
CNTAGCGA346630.16669513607801542No Hit
CATAGCGG304700.1465309060467106No Hit
CATAGCGT258500.12431322354143318No Hit
CATCGCGA252160.12126430347469165No Hit
CAGAGCGA245500.11806149469795685No Hit
CATAGCAA241210.11599842417960968No Hit
CGTAGCGA217540.10461546866229546No Hit
TATAGCGA217490.10459142355135902No Hit
CCTAGCGA216130.10393739653388764No Hit
CATGGCGA210110.10104236517713937No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)