FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19098964
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12550.085.485329
GTGCGAC31750.076.99171
CGCCGAA28900.076.733671
TGCGCGA29800.075.88981
GCGCTAG36100.073.999171
GACGCGT28550.073.8290941
ACGACCG29800.073.679421
TTTGCGC31200.073.188221
TTACCCG35750.073.085851
CGGTACG29350.069.572441
CCGCTAT35300.069.041581
TCCGCGA27750.068.9634558
AACGCGT30600.068.94923
GTTACGC27400.067.463328
GCGCGTA29550.067.1201551
CGAACGC37700.066.975951
ATAACGC36500.065.568621
TAAACCG43200.064.039691
CCTTACG34150.063.4369351
GGCGACT37050.063.4100341
Produced by FastQC (version 0.11.5)