FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19098964
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29050.0101.042696
ACGACGC29800.099.034835
CACGACG30750.095.50314
GACGCTC41800.071.262777
TACACGA45650.065.494752
CTACACG47050.063.035741
ACGCTCT49600.059.7638788
ACACGAC54750.054.3083273
CGCTCTT64700.045.4798369
TCTTCCG112650.06.317902610-14
CGTAGAG28301.3025783E-86.19505641
AACGGGG34401.0186341E-106.15828851
CTTCCGA135250.05.808775410-14
TAACCCT756350.05.7398436
CTAACCC807150.05.62380085
AACCCTA820600.05.51035551
CCTAACC833650.05.49719144
CCCTAAC833650.05.48054173
ACCCTAA835500.05.41986232
CGATGAG31105.6533645E-75.40241431
Produced by FastQC (version 0.11.5)