FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21090520
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGGAT2019539295.75578032215421No Hit
NGTCGGAT2222161.05362978248047No Hit
TGGCGGAT682860.3237758006914955No Hit
TGTCGGAG654980.3105565913026327No Hit
TGTCGGCT564430.2676226095895217No Hit
TGTCGGGT547210.2594578037905182No Hit
TNTCGGAT456170.21629149020507793No Hit
GGTCGGAT424720.20137957717495822No Hit
TGCCGGAT398090.18875305113387436No Hit
TGTCGGAA316720.15017173592685246No Hit
TGTCGGAC244760.11605214096191084Illumina NlaIII expression Adapter 1 (100% over 8bp)
TGTCGGTT226790.10753172515423992No Hit
AGTCGGAT219770.10420321547311302No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)