FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23126755
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2235355396.65667751485239No Hit
NGTCCAGA2335081.0096876972147628No Hit
GGGCCAGA556690.24071254268054468No Hit
GGTCCGGA433500.18744523388603374No Hit
GGTCCCGA397620.17193073563498207No Hit
GGTCCAGC395050.17081946861978692No Hit
GGTCGAGA325200.14061635538578587No Hit
GGCCCAGA302540.1308181800689288No Hit
GGTCCAGG299220.12938261334112805No Hit
TGTCCAGA282730.12225234365997305No Hit
GGTTCAGA238740.10323108451661289Illumina DpnII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)