FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23144756
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2223020196.0485433503814No Hit
NGTCCAGA2419291.0452864571136544No Hit
GGGCCAGA656100.2834767409083941No Hit
GGTCCAGG641370.2771124482798609No Hit
GGTCGAGA552130.2385551180578443No Hit
GNTCCAGA502090.2169346697800573No Hit
GGTCCAGC471330.20364440221361588No Hit
TGTCCAGA397480.17173652640796905No Hit
GGCCCAGA310100.13398283395167354No Hit
GGTCCCGA306800.13255702501249095No Hit
GGTCCAGT266260.11504117822629023No Hit
GGTTCAGA255590.11043106265626651Illumina DpnII expression Adapter 1 (100% over 8bp)
GGTCTAGA243900.10538024250504088No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)