FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20255930
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30500.083.5916751
TTTGCGC32300.082.811541
ACGACCG34100.076.495461
CGCCGAA31450.075.9121251
TTACGCG12500.075.3890159
CGGTACG33100.073.2410661
GCGCTAG36800.070.883021
CGAACGC37500.069.756361
GTTCGCG43350.069.0621268
ATAACGC37950.068.735051
TCACGCG30100.066.2285468
GTGCGAC35000.066.107141
CGAACTA41700.065.734541
CCGCTAT39500.064.545471
GGCGACT40550.064.327871
ACGCCGA41050.064.262351
GCGCGTA34400.064.261311
TAGGCGC36250.064.234131
CGCGGTA40050.063.659081
AACGCGT30200.063.1272853
Produced by FastQC (version 0.11.5)