FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20024728
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA32550.083.800981
TTACGCG13350.079.7417769
CGCCGAA30600.076.0322341
CGGTACG29600.075.890511
TTTGCGC32050.074.185331
GTGCGAC34400.072.721731
ACGACCG31700.071.553291
GCGCTAG32750.069.704611
GACGCGT30000.068.31461
TTACCCG38900.066.934051
CGAACGC35500.064.715861
GCGCGTA32200.064.326651
GTACGCT28400.063.4937258
ATAACGC37750.063.3702431
GGCGACT40900.063.1260261
AACCGCG35750.062.8352281
CCTTACG37550.062.7366141
CGAACTA40250.061.971031
AACGCGT28700.061.821333
TAGGCGC37800.061.3569561
Produced by FastQC (version 0.11.5)