FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19113365
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAATTC1830020695.74560000292989No Hit
NGCAATTC1991131.041747489256863No Hit
AGCAGTTC567140.29672430783381154No Hit
AGCACTTC457380.23929852226439458No Hit
AGCAATTA453500.23726852911562143No Hit
AGCAATTG446480.23359570645985153No Hit
ANCAATTC413790.21649249098732748No Hit
GGCAATTC359270.18796794808240203No Hit
AGGAATTC358980.18781622179035456No Hit
AGCAATTT329410.17234537194261712No Hit
AGCATTTC294960.154321334835598No Hit
AGCAAGTC283510.14833076227027528No Hit
CGCAATTC260900.13650134343167727No Hit
AGCAATGC237580.12430045677461816No Hit
AGCAATCC201410.10537652579752439No Hit
AGCCATTC197780.10347733117637842No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)