FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19874285
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12700.082.194039
TGCGCGA31400.081.705241
CGGTACG27800.075.984581
GTGCGAC39100.075.896491
ACGACCG28100.074.9132461
TTTGCGC32050.074.3467561
CGCCGAA32300.074.223891
GCGCTAG36600.072.693021
GACGCGT31250.070.402631
ATAACGC38350.069.757051
GCGCGTA34150.068.4907151
CGAACGC35600.066.727641
CCTTACG39100.065.6149441
ACGCCGA40050.065.518561
GTTACGC29500.064.6288
TTACCCG34850.064.598191
CCGCTAT35800.063.904841
TCACGCG28250.063.6385358
GTACCGT44650.063.4807748
CGTTGCG15450.062.4036677
Produced by FastQC (version 0.11.5)