FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21415162
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13750.084.352679
CGCCGAA35350.077.9372251
TTTGCGC35000.077.4638061
TGCGCGA32400.076.462351
CGGTACG31900.075.599031
GCGCTAG36800.071.4903951
GTGCGAC34900.070.5663151
ACGACCG30200.070.4172441
TCGTACG41500.068.997548
TTACCCG41350.066.8050461
GGCGACT41800.066.435521
GACGCGT34150.066.124251
GTTACGC33800.064.770148
GCGCGTA35100.063.9181561
GTACCGT46850.063.9035648
CCGCTAT43800.063.9024731
TCGACGG35950.063.2199971
TCGCGAT24950.062.1767238
TAGGCGC42300.062.0221861
TACGAAC48000.061.964961
Produced by FastQC (version 0.11.5)