FastQCFastQC Report
Wed 8 May 2024
EGAF00005486548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19026229
Total Bases152.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGATCTA1834783996.43444846585206No Hit
NGGATCTA1971031.0359541031488688No Hit
AGGAGCTA443170.23292582045554064No Hit
ANGATCTA401330.21093512539978365No Hit
AGGGTCTA367210.19300198688873135No Hit
AGGCTCTA326320.17151060254767247No Hit
AGGATGTA300770.15808177227342318No Hit
AGGATCTC283560.14903636448399732No Hit
CGGATCTA278400.14632431891784758No Hit
GGGATCTA263620.1385560953775969No Hit
AGGTTCTA235530.12379226592931264No Hit
AGGATCAA202330.10634267042617851No Hit
TGGATCTA196040.103036707904651No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)