FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001999
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30700.079.654111
TTACGCG12950.079.494939
CGCCGAA35000.077.7933651
GTGCGAC33900.073.427111
ACGACCG31650.072.189231
CGGTACG30700.072.0453641
TTTGCGC31950.071.054451
TTACCCG42400.068.520251
CGAACTA48100.066.470791
GACGCGT33200.065.740781
CGAACGC40100.065.715011
TCCGCGA33000.064.588358
TAGGCGC40050.064.521211
TCGTACG42300.064.099118
CGTTGCG16550.063.516227
ATAACGC38900.063.4262241
TCACGCG29950.063.419868
AACCGCG38500.063.1371881
ACGCCGA41350.063.0223271
TACGAAC43150.062.423391
Produced by FastQC (version 0.11.5)