FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20600131
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11800.082.8529
TTTGCGC33650.074.977411
ACGACCG32750.074.366021
CGCCGAA33100.072.918781
CGGTACG31450.072.802851
TGCGCGA32400.072.6937261
GCGCTAG33000.071.372021
GTGCGAC36100.071.100861
ATAACGC37550.070.879771
CGAACGC38850.069.8218461
CCGCTAT41450.067.729141
TCGTACG36350.066.3423848
TAGGCGC37600.065.9371951
GACGCGT32750.065.682561
GTACGCT30150.063.8918658
TTCCGCG18150.063.840417
GGCGACT42700.063.69721
ACGCCGA42150.063.4903761
TCGCGAT23550.063.039718
GCCGGAT38700.062.932491
Produced by FastQC (version 0.11.5)