FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20302912
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11700.087.36079
TGCGCGA31300.080.3134161
CGCCGAA31300.076.110971
TTTGCGC30500.074.034251
CGGTACG31850.073.878891
GTGCGAC31900.071.701391
GCGCGTA35900.069.004791
ATAACGC36700.067.6997151
ACGACCG29300.067.588941
GACGCGT30500.067.0860441
CGAACGC35900.066.9692461
GTTACGC29750.066.765128
TTACCCG40200.066.713541
GCGCTAG33850.066.4914861
CCGCTAT41000.066.3029861
GGCGACT38550.066.156881
ACGCCGA39850.064.73221
TCGCGAT23600.064.5050058
GTACCGT43950.063.0072568
CGCGGTA38450.062.9079671
Produced by FastQC (version 0.11.5)