FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19680284
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC33150.0104.751735
CGACGCT33250.0103.783136
CACGACG35850.097.469144
GACGCTC46250.075.08197
TACACGA51200.069.6893842
CTACACG52200.067.6886441
ACGCTCT51000.067.662878
ACACGAC57250.061.6690333
CGCTCTT66000.052.7245869
TCTTCCG122250.06.70142710-14
CTTCCGA142900.06.08816910-14
CGTAGAG26701.7279126E-76.0152321
TAACCCT827250.05.88026956
CTAACCC885100.05.7498175
CCCTAAC915150.05.65613273
CCTAACC908900.05.6471124
CGATGGG36302.4356268E-95.63108871
AACCCTA895800.05.60684251
ACCCTAA920950.05.58318042
AACGGGG33709.037467E-85.41565561
Produced by FastQC (version 0.11.5)