FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20410748
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11800.088.4672249
TGCGCGA32100.076.899891
ACGACCG31450.074.077121
CGGTACG34700.073.663541
TTTGCGC33100.073.473431
CGCCGAA31200.072.798061
GCGCTAG36200.071.418171
CCGCTAT40750.069.178911
GACGCGT33500.068.2361
GTGCGAC38800.067.5735551
GCGCGTA34400.066.875371
CGAACGC38700.065.106171
ATAACGC37050.064.0632861
AACCGCG38050.063.915121
GTTCGCG45950.063.7382248
GTACCGT47800.063.2429738
CGCGGTA40300.063.064981
ACGCCGA42650.062.8436051
GGCGACT41000.062.3445131
TCGTACG38750.062.2983638
Produced by FastQC (version 0.11.5)