FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001999
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGGAT2033098196.80498032592041No Hit
NGTCGGAT1594220.7590801237539341No Hit
TGTCGGGT575930.2742262772224682No Hit
TGTCGGAG552300.26297496728763775No Hit
TGTCGGCT531820.2532235145806835No Hit
TGGCGGAT462750.2203361689522983No Hit
TGTCGGAA393220.18722979655412803No Hit
TGCCGGAT347780.1655937608605733No Hit
TGTCGGAC248480.1183125472960931Illumina NlaIII expression Adapter 1 (100% over 8bp)
GGTCGGAT219760.10463765853907525No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)