FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19582824
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11200.084.139599
TGCGCGA30150.081.653631
CGCCGAA29350.079.107711
ACGACCG27750.077.559721
TTTGCGC30500.073.708291
GCGCTAG31700.073.010741
GTGCGAC33000.071.9214251
CGGTACG30550.070.451131
TCGTACG37650.068.7350548
CGTTGCG15750.067.6566857
ACGCCGA37350.066.702551
ATAACGC36950.066.626721
GCGCGTA31400.065.257671
TAGGCGC36200.064.342051
GACGCGT30350.063.8724251
GGCGACT37700.063.150611
TCACGCG27050.062.976088
GTACCGT44450.062.7861758
GTACGCT30550.062.1677448
AACGCGT26850.062.0935333
Produced by FastQC (version 0.11.5)