FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16624613
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT25500.0105.185726
ACGACGC26500.0102.8577965
CACGACG28750.095.3123554
GACGCTC35650.075.035147
CTACACG41200.067.303411
TACACGA41750.066.243832
ACACGAC46050.059.9760063
ACGCTCT45250.059.7560128
CGCTCTT59150.045.7153859
CGCAAAG25106.9867383E-96.7042351
AACGGGG31456.91216E-116.51377151
CGCCAAT13650.00155651176.43196541
ACGGGAG39350.06.32163331
GTTCCGA48350.06.1472755
TCTTCCG104000.05.96698910-14
CTTCCGA121600.05.508752310-14
CTAACCC646200.05.4745465
TAACCCT620000.05.46034966
CCCTAAC667050.05.36847263
CCTAACC668100.05.3167954
Produced by FastQC (version 0.11.5)