FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20304613
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1948667995.9716838730194No Hit
NAGTAGAG1960730.9656574099688577No Hit
AAGGAGAG851870.4195450560914409No Hit
AAGTGGAG509910.2511301249622438No Hit
AAGTCGAG507500.24994320256190058No Hit
AAGTTGAG371880.18315049885461987No Hit
ACGTAGAG360430.17751138620568638RNA PCR Primer, Index 17 (100% over 8bp)
AGGTAGAG345590.17020270221353148No Hit
GAGTAGAG334290.1646374644027936No Hit
AAGAAGAG328650.1618597704866377No Hit
AAGTAGCG323490.15931847605270782No Hit
CAGTAGAG276310.13608237694557387No Hit
AAGCAGAG267680.13183211125471833No Hit
AAGTAGGG258910.12751289571488017No Hit
AAGTAGAA240940.11866269009904301No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)