FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20847149
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12800.088.913969
TGCGCGA32050.079.3457261
CGGTACG35150.078.792741
TTTGCGC34500.078.582731
CGCCGAA32450.075.4401251
ACGACCG32350.075.221541
GTGCGAC36300.071.666421
GACGCGT32650.071.396971
GCGCTAG37800.071.142371
AACCGCG37050.069.8212051
GCGCGTA36750.067.806191
CCGCTAT41100.066.497011
GGCGACT38300.066.397661
CGAACGC36800.065.331161
GTTCGCG47400.064.844648
TCGTACG37300.064.717548
CGTTGCG18550.064.480397
TAGGCGC38300.064.298881
GTACCGT47700.064.2848368
ATAACGC39550.063.3752861
Produced by FastQC (version 0.11.5)