FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20847149
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG2003921896.12450124475053No Hit
NAGTAGAG2150311.0314647820668428No Hit
AAGGAGAG737080.3535639333704575No Hit
AAGTCGAG486460.2333460560962077No Hit
AAGTGGAG473740.22724450235377508No Hit
ANGTAGAG440810.2114485774529649No Hit
AGGTAGAG373320.1790748461576209No Hit
ACGTAGAG349160.16748573150218285RNA PCR Primer, Index 17 (100% over 8bp)
AAGTTGAG331320.15892820644204153No Hit
AAGTAGCG317820.15245250081917677No Hit
AAGAAGAG289560.1388966903819798No Hit
AAGTAGGG276320.13254570205259242No Hit
AAGCAGAG256920.12323987323158672No Hit
AAGTAGAA253950.12181521799455647No Hit
GAGTAGAG229880.11026927470993755No Hit
CAGTAGAG221640.10631669587050009No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)