FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20261956
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC36000.079.079641
ACGACCG29350.078.343871
TTACGCG11050.078.073549
TGCGCGA34050.077.177021
CGCCGAA32650.075.567681
GTGCGAC37750.073.673381
CGGTACG33500.072.560791
GCGCTAG33350.071.573871
CGAACGC37850.070.393011
CGTTGCG16150.065.986557
TAGGCGC38650.065.158661
TCGTACG36750.064.901958
GTACCGT45950.064.687078
CCGATAA41750.064.34191
TCGCGAT24250.064.2755668
AACCGCG39750.063.906451
ATAACGC36200.063.5191351
CTTACCG42550.063.47531
CCGCTAT38850.063.3200841
GTTCGCG41800.063.1312378
Produced by FastQC (version 0.11.5)