FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19269490
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11350.081.1186759
TGCGCGA29200.080.3002241
ACGACCG29850.078.796361
GTGCGAC33750.077.2661061
GCGCTAG35400.073.0456851
CGCCGAA28700.071.2640841
CGGTACG29400.070.312681
TTTGCGC32050.069.513061
GACGCGT30550.067.187681
GCGCGTA32200.067.147581
CGTTGCG13550.066.343057
AACGCGT27000.065.514073
TTACCCG35800.064.6801151
CGAACGC34900.064.673681
GTACCGT39850.064.582238
CGAACTA40800.063.5569081
CCGCTAT35200.063.2924271
GGCGACT36200.062.9565051
CCTTACG37250.062.946751
ATAACGC34900.062.1620861
Produced by FastQC (version 0.11.5)