FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19123270
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG8800.087.322599
TGCGCGA28900.080.851991
CGGTACG29550.078.08511
ACGACCG29700.077.444881
TTTGCGC31850.076.3437351
CGCCGAA30800.074.441921
GTGCGAC33650.074.429931
GCGCGTA30250.070.243511
GCGCTAG34800.069.662231
GACGCGT31150.068.448411
CCTTACG35800.066.696541
GTTCGCG38650.065.273458
GGCGACT34950.064.975821
GCCGGAT38900.064.572471
CCGCTAT38150.064.3107151
TCCGCGA27850.063.7745978
AACCGCG34200.063.6251371
GTCGGAC30700.063.267671
TTACCCG36800.061.9078031
CGAACGC35000.061.7535971
Produced by FastQC (version 0.11.5)