FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19123270
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29500.099.0358056
ACGACGC31250.094.415755
CACGACG33100.090.012684
GACGCTC42700.068.590127
TACACGA46350.066.034852
CTACACG47700.064.7418061
ACGCTCT51700.056.6498648
ACACGAC55750.054.22173
CGCTCTT65850.044.4766929
CGTTGAG26656.184564E-117.1564431
CCGGTAG14253.3683976E-46.6919021
CGTTAGG22001.9213257E-76.66850381
TCTTCCG113950.05.98006810-14
CGTAAAG24751.5977439E-65.9275591
CGGGTTA26259.1013135E-75.79935744
TAACCCT776600.05.79700146
CGCTAGG16550.00204804475.76190951
CGCTTAG15300.00541951045.75321871
GTTCCGA50050.05.6488585
CTAACCC824500.05.61836965
Produced by FastQC (version 0.11.5)