FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17973784
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC29500.081.770351
TGCGCGA26700.077.478131
ACGACCG28000.076.491561
TTACGCG11300.075.056159
GTGCGAC32750.074.3253251
CGCCGAA29700.073.343851
CGGTACG29100.071.590561
GACGCGT25200.069.616891
GCGCTAG30750.068.699991
ATAACGC32550.067.595761
TTACCCG31450.066.706041
GCGCGTA29550.066.542441
CCGCTAT34900.065.767011
CGAACGC34700.065.09281
AACGCGT24050.064.822833
TAGGCGC35450.063.9218561
CGCGGTA34900.063.4630781
TCGTACG35550.063.2129178
GGCGACT38650.062.9794241
ATTAGCG37300.062.7111931
Produced by FastQC (version 0.11.5)