FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17973784
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26600.0100.3124546
ACGACGC27750.095.875655
CACGACG29700.089.578294
GACGCTC37450.071.446167
TACACGA43800.062.169462
CTACACG45800.058.854581
ACGCTCT47250.056.474088
ACACGAC49850.053.9512983
CGCTCTT60000.044.715049
AACGGGG34000.08.8328911
TCTTCCG105500.06.157436410-14
CGAAAAG29553.0771844E-85.94910671
TAACCCT496300.05.66860346
CGCTGAG35608.40555E-95.55535361
GTTCCGA48651.8189894E-125.51345875
CTTCCGA122450.05.482726610-14
ACCGGGT24053.8938575E-55.48220631
CTAACCC514100.05.37257675
AACCCTA546350.05.2688951
CCCTAAC542050.05.2023963
Produced by FastQC (version 0.11.5)