FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20261956
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1953426496.40857970474322No Hit
NAGTAGAG1521530.7509294759104205No Hit
AAGGAGAG718790.3547485741258149No Hit
AAGTCGAG484240.23898975992248725No Hit
AAGTGGAG462350.22818626197786632No Hit
AAGTAGAA375920.18552996561635018No Hit
AGGTAGAG357550.17646371357237178No Hit
ACGTAGAG340880.16823647233268102RNA PCR Primer, Index 17 (100% over 8bp)
AAGTTGAG329590.16266445352067688No Hit
AAGTAGCG321380.158612524871735No Hit
AAGAAGAG315440.15568092241440068No Hit
AAGTAGGG288300.1422863616918327No Hit
AAGCAGAG252770.12475103588222183No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)