FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19032986
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11850.086.2610869
TTTGCGC29750.077.5317461
CGGTACG28850.077.420341
GTGCGAC32800.075.440631
CGCCGAA29800.074.70731
TGCGCGA27900.074.03921
ACGACCG26150.073.690531
GACGCGT31350.070.082311
CGAACGC34050.068.3837661
GTTCGCG38450.066.5568168
GCGCTAG34950.065.1608661
CGAACTA43850.064.91941
TAGGCGC34300.064.267631
ATAACGC35600.064.176171
GTTACGC29300.063.8360868
TTACCCG36150.063.199771
GCCGGAT40050.062.6953541
CCGCTAT36600.061.6249921
TCGTACG34150.061.563248
GCGCGTA31150.061.3935471
Produced by FastQC (version 0.11.5)