FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20794248
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC34600.081.9214861
CGCCGAA29950.080.005381
TTACGCG11850.077.69619
TGCGCGA31100.076.577181
GTGCGAC37800.072.280641
CGGTACG30350.071.489111
ACGACCG30400.070.890921
GCGCTAG36550.068.956291
TTACCCG37250.067.072121
GTACGCT28450.067.017368
TAGGCGC38700.067.013081
TCACGCG32200.066.867398
GGCGACT41700.066.221341
GACGCGT33150.065.891561
CCGCTAT39850.065.079191
GCCGGAT36950.064.2556151
TCGTACG42450.063.7008068
CGCGGTA38350.063.243361
CCTTACG41850.063.0164451
CGAACGC38300.062.3722151
Produced by FastQC (version 0.11.5)