FastQCFastQC Report
Wed 8 May 2024
EGAF00005486635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20255930
Total Bases162 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1932837195.42080269827157No Hit
NAGTAGAG2099281.0363779890629559No Hit
AAGTGGAG730380.3605758906157357No Hit
AAGGAGAG704930.34801166868171446No Hit
AAGTCGAG544010.2685682661818045No Hit
AGGTAGAG495680.24470858657193226No Hit
ANGTAGAG434060.21428786533128816No Hit
AAGTTGAG424130.2093855972053616No Hit
ACGTAGAG400540.19773962489009392RNA PCR Primer, Index 17 (100% over 8bp)
GAGTAGAG386920.19101566800438194No Hit
AAGTAGCG332010.16390755694752104No Hit
AAGAAGAG313830.1549324074480905No Hit
AAGTAGAA302020.14910201605159576No Hit
CAGTAGAG279920.13819163079651242No Hit
AAGTAGGG260370.12854013614778487No Hit
AAGCAGAG252600.12470422241783023No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)