FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20458078
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1961764695.89193080601218No Hit
NAGTAGAG2315071.1316165673041232No Hit
AAGGAGAG766150.37449754566386934No Hit
AAGTGGAG671960.32845705251490387No Hit
AAGTCGAG550460.269067309255542No Hit
AAGTTGAG372130.18189880789387938No Hit
AGGTAGAG344100.16819761856416815No Hit
ANGTAGAG336790.16462445787918104No Hit
ACGTAGAG319350.15609970790022407RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGCG296550.1449549659552574No Hit
AAGAAGAG296090.14473011589847296No Hit
AAGTAGGG280710.13721230313033317No Hit
AAGCAGAG255620.12494819894615711No Hit
AAGTAGAA240030.1173277372390505No Hit
GAGTAGAG224790.10987835709688858No Hit
CAGTAGAG223210.10910604603228122No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)