FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19564791
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTGAC1892103196.70959940231408No Hit
NACTTGAC1896870.96953246267747No Hit
GACTTGAC442590.22621759670215746No Hit
CACTTGAC397900.203375543342119No Hit
TACTTGAC335720.1715939618266303No Hit
AACTTGCC318240.16265954489368173No Hit
AGCTTGAC276740.14144797151168137No Hit
AACTTGGC236400.1208292999398767No Hit
ACCTTGAC227380.11621897724335517No Hit
AACGTGAC215190.10998839701379891TruSeq Adapter, Index 21 (100% over 8bp)
AACTTGAA203480.10400315546432364No Hit
AAGTTGAC202670.10358914644168701No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)